Canine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:48 wells The microplate provided in this

The microplate provided in this kit has been precoated with anti HAS2 antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF2

Substrate solution is then added and color develops in proportion to the amount of Human RHPN2

Calculate the concentration of IARS in the sample by plotting standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated LEP antibody

Canine TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:48 wells The microplate provided in thisThe Canine TNF Alpha (Tumor Necrosis Factor Alpha) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Canine TNF Alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine TNF Alpha and Avidin Horseradish Peroxidase (HRP) conjugate are added