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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPX
Then Streptavidin-HRP is added and binds to the Biotinylated AIF1 antibody
And then biotinylated Human P3H1 Antibody is added and binds to P3H1 in the sample
Substrate solution is then added and color develops in proportion to the amount of Mouse UMOD
The concentration of Goat LEP in the samples is then determined by comparing the OD of the samples to the standard curve
Human SMPDL3B (Acid Sphingomyelinase-like Phosphodiesterase 3B) ELISA Kit YYL-4 Standards or samples are addedThe Human (SMPDL3B) Acid Sphingomyelinase like Phosphodiesterase 3B ELISA Kit measures Acid Sphingomyelinase Like Phosphodiesterase 3B in samples. The plate has been pre coated with Human SMPDL3B antibody. SMPDL3B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SMPDL3B Antibody is added and binds to SMPDL3B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SMPDL3B
